Chapter 1423: Chapter 1067: Of the Weak Water Three Thousand, I Scoop Only One Ladleful Chapter 1423: Chapter 1067: Of the Weak Water Three Thousand, I Scoop Only One Ladleful Sisi’s recovery was going well, and after evaluation, Yang Ping felt it was possible to experiment with the non-castrated virus. Although there were certain risks, he decided to give it a try.

If one always hesitates to take risks, it is difficult to achieve breakthroughs. It is feasible to take risks after thorough assessment and preparation.

Even if it doesn’t succeed, just consider it a severe cold. Yang Ping was actively preparing for the next experiment.

The virus laboratory at Nandu Medical University began a new round of various tests on the K virus. After several rigorous tests, the new K virus was considered to have not lost the basic characteristics of the original virus, ensuring the safety of experiments using replicable viruses.

The Tumor Laboratory at Sanbo Hospital also concluded after a series of checks that the K virus mainly infects tumor cells and a smaller portion of normal cells. However, observations from several experiments show that it only causes apoptosis in tumor cells, not in normal cells. Its impact on normal cells is lower than that of its source virus–the adenovirus–on human cells.

The vector source virus used by the K virus is a type of adenovirus–but not just any adenovirus, rather a recombinant adenovirus. Recombinant adenovirus, a viral vector system with replication defects, is widely used in fields such as gene therapy and basic life science research.

Recombinant adenovirus has several notable advantages: broad infection range, capable of infecting all cell lines, primary cells, and some tissues; infection efficiency up to 100%, surpassing all other viral vector tools and liposome transfection; large external gene capacity, capable of carrying up to 8Kb; does not integrate into the genome; high titer, easy to handle. Therefore, recombinant adenovirus has always been regarded as one of the most promising gene delivery tools.

Of course, Yang Ping borrowed it not to deliver genes, but to deliver the K factor. Before using it, Yang Ping further refined it. He then embedded the K factor into its protein structure to form a protein complex.

The commonly used adenovirus vector is based on human adenovirus type 5, whose genome is a 36Kb long linear double-stranded DNA. This type of adenovirus enters the cell by binding its own fibers to cellular surface receptors (such as CAR), then is endocytosed from the endosome to the cytosol and nucleus, initiating viral replication and assembly with the help of cellular transcription and translation machinery; a complete viral life cycle leads to cell death and the release of viral particles.

The ordinary adenovirus is also not to be taken lightly. Once infected, it can easily cause diseases in the respiratory, digestive, and urinary tracts, such as pharyngitis, pneumonia, conjunctivitis, etc., resulting in symptoms like fever, cough, sputum, throat redness, swelling, congestion, diarrhea, vomiting, etc., and in severe cases, can cause breathing difficulties leading to suffocation death.

However, recombinant adenovirus is completely different from the ordinary adenovirus, with differences similar to those between dogs and wolves. Recombinant adenovirus has been completely domesticated by researchers; not only have its replication genes been knocked out, but some of its pathogenic genes have also been eliminated on the basis of understanding its genes and characteristics. Hence, this recombinant virus no longer has strong pathogenicity, but retains other useful characteristics for experiments, such as: a broad infection range, high infection efficiency, and following Yang Ping’s modification, a very high infection rate for undifferentiated tumor cells compared to normal cells.

Therefore, the adenovirus recombinantly modified by Yang Ping has tremendous potential. It is a significant innovation in itself, not only for use in K virus therapy but also for many other tumor treatments in the future. It adds a highly energetic and precise weapon to the arsenal for treating tumors.

Even so, Yang Ping does not dare to take this lightly. He meticulously reexamined the genes of these viruses to confirm that their genomes were correct.

This specially recombinant adenovirus has one more significant advantage over the original ordinary adenovirus.

The original ordinary adenovirus possesses strong infectivity, as it can survive for 30 minutes in an environment at 56degC and two minutes at 100degC, which facilitates its transmissibility.

However, the adenovirus recombinantly modified by Yang Ping’s team can survive for no more than one minute in dry conditions, with an average life span of only about twenty seconds. Even in moist environments, it does not survive more than one minute at 56degC. Thus, this virus is extremely fragile. It cannot survive outside the body and can only exist inside the human body. Additionally, the human immune system maintains sufficient lethality against it, which makes it far less threatening to humans than the ordinary adenovirus.

So no matter how you look at it, this recombinantly modified adenovirus is very safe. Therefore, Yang Ping’s risk-taking is not reckless; he has enough confidence to undertake this.

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The K virus cultured in the virology laboratory of Nandu Medical University had completed packaging and was transported cold-chain to Sanbo Hospital. Yang Ping injected these viruses into Sisi’s body.

Clearly, the live virus had a much greater impact on the human body than the castrated virus. On the third day after the virus was injected, Sisi developed a high fever again, but this time the temperature was not so exaggerated, only 39 degrees. For Sisi, who had already gone through several high fever tests of 42 degrees, she could completely withstand a fever of 39 degrees.